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产KPC细菌检测的新方法评估

时间:2020-07-21 来源:
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 产KPC细菌检测的新方法评估  

基于显色培养基和MIC梯度定量试纸的组合检测






筛查培养基


美德声利飞驰系列CRE显色培养基,用来检测耐碳青霉烯的肠杆菌科细菌。所有平板在36 +/- 1°C需氧条件下孵育,在18-24小时后读取结果,并根据说明书进行结果解释。
 


肺炎克雷伯菌KPC阳性




MIC梯度定量试纸


MTS厄他培南/厄他培南+硼酸是由厄他培南(0.125-8 mg/L)和厄他培南(0.032-2 mg/L)加固定浓度硼酸的双边梯度定量试纸。MTS美罗培南/美罗培南+硼酸是由美罗培南(0.125-8 mg/L)和美罗培南(0.032-2 mg/L)加上固定浓度的硼酸的双边梯度定量试纸。


所有试纸条均接种在MH琼脂平板上。孵育24小时后进行结果解释。所有菌株均在CRE显色培养基上,并在MH琼脂平板上用MTS厄他培南/厄他培南+硼酸和MTS美罗培南/美罗培南+硼酸进行敏感性和特异性检测。


MTS厄他培南/厄他培南+硼酸和MTS美罗培南/美罗培南+硼酸





REFERENCES


[1] K.F.Anderson, D.R.Lonsway, J.K.Rasheed, J.Biddle, B.Jensen, L.K.McDougal, R. B.Carey, A.Thompson, S.Stocker, B.Limbago and J.B.Patel. 2007

Evaluation of Methods to Identify the Klebsiella pneumoniae Carbapenemase in Enterobacteriaceae.

Journal of Clinical Microbiology. Vol.45, N.8: 2723-2725.


[2]A.Tsakris,I.Kristo, A.Poulou, K. Themeli-Digalaki, A.Ikonomidis, D.Petropoulou, S.Pournaras, D.Sofianou .2009

Evaluation of boronic acid disk tests for differentiating KPC-possessing Klebsiella pneumoniae isolates in the clinical laboratory.

Journal of Clinical Microbiology. Vol.47, N.2: 362-367.


[3] D.Landman, J.Salamera, M.Singh, J.Quale. 2011.

Accuracy of Carbapenem Nonsusceptibility for Identification of KPC-Possesing Enterobacteriaceae by Use of the Revised CLSI Breakpoints.

Journal of Clinical Microbiology. Vol.49, N.11: 3931-3933.


[4] Wolter DJ, Kurpiel PM, Woodford N, Palepou MF, Goering RV, Hanson ND.

Phenotypic and enzymatic comparative analysis of the novel KPC variant KPC-5 and its evolutionary variants, KPC-2 and KPC-4.

Antimicrob Agents Chemother. 2009 Feb;53:557-62.


[5] Papp-Wallace KM, Bethel CR, Distler AM, Kasuboski C, Taracila M, Bonomo RA.

Inhibitor resistance in the KPC-2 beta-lactamase, a preeminent property of this class A beta-lactamase.

Antimicrob Agents Chemother. 2010 Feb;54:890-7.





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